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appropriate isotype controls for af647  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc appropriate isotype controls for af647
    Appropriate Isotype Controls For Af647, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/appropriate+control+igg/Rabbit+mAb+IgG+XP+Isotype+Control/pmc12829025-46-0-6
    Average 94 stars, based on 61 article reviews
    appropriate isotype controls for af647 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation.
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90 μL Dynabeads Protein G (Invitrogen) while constantly rotating, and prior to cell harvesting. ..

    Article Title: Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90 μL Dynabeads Protein G (Invitrogen) while constantly rotating, and prior to cell harvesting. ..

    Article Title: An extra-glycolytic function for hexokinase 2 as an RNA-binding protein regulating SOX10 mRNA translation in melanoma
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap® Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90μl Dynabeads Protein G (InvitrogenTM) while constantly rotating, and prior to cell harvesting. ..

    Control:

    Article Title: Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation.
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90 μL Dynabeads Protein G (Invitrogen) while constantly rotating, and prior to cell harvesting. ..

    Article Title: Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90 μL Dynabeads Protein G (Invitrogen) while constantly rotating, and prior to cell harvesting. ..

    Article Title: An extra-glycolytic function for hexokinase 2 as an RNA-binding protein regulating SOX10 mRNA translation in melanoma
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap® Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90μl Dynabeads Protein G (InvitrogenTM) while constantly rotating, and prior to cell harvesting. ..

    Cell Harvesting:

    Article Title: Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation.
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90 μL Dynabeads Protein G (Invitrogen) while constantly rotating, and prior to cell harvesting. ..

    Article Title: Hexokinase 2 is an RNA-binding protein that regulates mRNA translation independently of glycolysis and induces melanoma cell proliferation
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90 μL Dynabeads Protein G (Invitrogen) while constantly rotating, and prior to cell harvesting. ..

    Article Title: An extra-glycolytic function for hexokinase 2 as an RNA-binding protein regulating SOX10 mRNA translation in melanoma
    Article Snippet: The rest of the lysate was incubated with the HK2 antibody-conjugated beads or GFP-Trap® Magnetic Agarose (ChromoTek, #gtma) overnight at 4°C while constantly rotating. .. For endogenous HK2 immunoprecipitation, 9 μg of HK2 antibody (Abcam, ab209847) or appropriate control IgG (rabbit; Cell Signaling, #2729S) were coupled overnight at 4°C to 90μl Dynabeads Protein G (InvitrogenTM) while constantly rotating, and prior to cell harvesting. ..



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